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WELCOME

FROM BENCHSIDE TO BLADESIDE

The SurgiCAL ProtEomics Laboratory (SCALPEL) was commissioned in February 2018 in the Institute for Health Innovation and technology (iHealthtech), NUS. Using a multidisciplianry approach, our aim is to translate scientific breakthroughs into tangible improvements in patient outcomes. Using a combination of mass spectrometry imaging, proteomics and primary patient derived organiod culture, our research is targeted towards improving the efficacy, safety and precision of chemotherapy, as well as development of mass spectrometry based platforms for real-time pathological and surgical analysis with an emphasis on pancreatic ductal adenocarcinoma (PDAC) and hepatocellular carcinoma (HCC).

OUR RESEARCH

MASS SPECTROMETRY IMAGING

Mass spectrometry imaging (MSI) allows for spatial resolution of mass spectra directly overlaid on tissue sections, allowing for visualization of molecular distribution at extremely high resolutions.

PRECISION MEDICINE

By culturing tumour tissues from biopsies and resections into organoids, we are able to perform patient specific in-vitro chemotherapeutic drug screening and proteomic biomarker discovery.

CANCER VACCINES

Using our ability to culture Patient Derived Organoids (PDOs), we are able to expand MHC class I positive lines. Using LC-MS analysis, we are then able to conduct neoantigen discovery by isolating and identifying bound peptides using LC-MS analysis.

INDUSTRY PARTNERSHIP

In 2019, SCALPEL signed a landmark research collaboration agreement with Bruker, giving us access to state-of-the art mass spectrometry (MS) technologies such as rapifleX®, nanoElute and maXis Plus for the development of MS tissue imaging and proteomics platforms.

SERVICES

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MALDI IMAGING

MALDI imaging is mass spectrometry driven molecular histology, able to directly overlay MS profiles in pixels over tissue. making it versatile solution for visualizing distribution of targeted compounds, or for spatial biomarker discovery

MALDI MOLECULAR WEIGHT DETERMINATION

Matrix assisted laser desorption ionization (MALDI) is a soft ionization technique allowing for ionisation without fragmentation and coupled with TOF/TOF MS, can detect and identify a wide range of biomolecules.

SHOTGUN PROTEOMICS

Proteins are the drivers of a cells function. Through the use of proteomics technology to study all proteins in a biological system, we are able to get a global snapshot of the state of the system. this makes it an ideal technique for many applications such as unbiased biomarker discovery.

CONTACT US

MD6 #14-02 – Centre for Translational Medicine
Yong Loo Lin School of Medicine
National University of Singapore
Singapore 117599

yf_lee@nus.edu.sg