SERVICE WORKFLOW OVERVIEW
OUR SERVICES
MATRIX ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY IMAGING
Matrix assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI) is mass spectrometry driven molecular histology. By capturing the mass spectrum profiles on its surface in discrete pixels, MALDI-MSI preserves the molecular spatial context of your tissue section. It is a proven technology, capable of label free and multiplexed analysis of lipids, metabolites, peptides, proteins on tissue sections, and even polymer in industrial setting.
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Coupled with extensive downstream univariate and multivariate statistical analysis on thousands of mass spectra on each sample surface, this technique is a versatile solution for visualizing distribution of targeted compounds, or for spatial biomarker discovery. MALDI-MSI can be combined with other -omics or histological analysis that provide compound identification, quantification and context at bulk or single cell level, enabling deep probing into complex biological interactions on a micro and macroscopic scale.
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Scope of Services:
Cyrosectioning – Frozen tissue
On-slide tissue digestion – Frozen tissue
FFPE sample preparation
Matrix deposition
MALDI-TOF – Reflectron Mode
MALDI-TOF – Linear Mode
MALDI-MSI Post-Run Analysis: Basic
PDI-MSI Post-Run Analysis: Extended
MALDI MOLECULAR WEIGHT DETERMINATION
Matrix assisted laser desorption ionization (MALDI) is a soft ionization technique. Similar to electrospray ionisation (ESI), MALDI enables ionization of large molecules like proteins without extensive fragmentation but unlike ESI, MALDI ions tend to be singly charged. Therefore, MALDI mass spectrum is fairly uncomplicated and simple to decipher.
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MALDI is a discrete sample analysis method, requiring samples to be placed on separate sample spots which can be analysed multiple times and has fairly durable shelf life. Coupled with TOF/TOF MS, MALDI can detect intact or digested proteins, peptides, lipids, metabolites, polymers, and have been used to directly mass profile various biological samples, such as bacteria samples and viral particles.
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Scope of Services:
Molecular Weight Analysis
PROTEOMICS
Proteomics is the application of technologies to study all proteins in a biological system (e.g., cell, tissue), including the expression, interactions, structure, functions and modifications at any stage. Proteins are effectors of biological functions, so qualitative or quantitative changes in the highly dynamic proteome, provides a snapshot of the immediate environment in which it is examined.
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MS-based bottom-up proteomics technologies, coupled with bioinformatic tools play crucial roles in enabling identification of proteins by comparing measured masses of digested protein peptides to calculated masses obtained from genomic data. These techniques can be utilized broadly for different research settings, such as unbiased biomarker discovery, targeted vaccine development, multiplexed quantitative protein profiling, cellular stress response to drugs, functional mapping of protein complexes and insights into pathogenicity mechanisms in different diseases.
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Scope for Services:
Tissue protein extraction
In-gel protein sample preparation
In-solution multienzyme digestion(MED)- filter assisted sample preparation(FASP)
Reverse-phase HPLC sample fractionation
TMT 6-plex quantitative proteomics sample preparation
Proteomics LC ESI-MS
Proteomics Post-Run Analysis: Basic
Proteomics Post-Run Analysis: Extended
GET A QUOTE
For service request, please complete our MS Services Request Form and submit to Ms. Lee Yi Fang at yf_lee@nus.edu.sg or fill up the online form below.
